{"id":4131,"date":"2020-04-23T14:24:50","date_gmt":"2020-04-23T12:24:50","guid":{"rendered":"https:\/\/www.prager-elektronik.at\/?post_type=product&#038;p=4131"},"modified":"2024-09-11T14:23:48","modified_gmt":"2024-09-11T12:23:48","slug":"openspr","status":"publish","type":"product","link":"https:\/\/www.prager-elektronik.at\/en\/produkte\/life-science\/localized-surface-plasmon-resonance-lspr\/openspr\/","title":{"rendered":"OpenSPR"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;]<div class=\"title-wrapper  basel-title-color-default basel-title-style-default basel-title-size-small text-left \"><div class=\"liner-continer\"> <span class=\"left-line\"><\/span> <h4 class=\"title\" >Nicoya OpenSPR<span class=\"title-separator\"><span><\/span><\/span><\/h4> <span class=\"right-line\"><\/span> <\/div><\/div>[vc_column_text]<strong>Publication-quality binding kinetics &amp; affinity data on your benchtop.<\/strong><\/p>\n<p>OpenSPR is the world\u2019s only benchtop surface plasmon resonance (SPR) instrument. It provides high quality, label-free interaction analysis for a fraction of the cost of existing solutions. Our unique nano-structured sensor surface uses localized SPR (LSPR) to deliver repeatable, highly sensitive kinetic data.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"title-wrapper  basel-title-color-default basel-title-style-default basel-title-size-small text-left \"><div class=\"liner-continer\"> <span class=\"left-line\"><\/span> <h4 class=\"title\" >Infos \/ Datasheet<span class=\"title-separator\"><span><\/span><\/span><\/h4> <span class=\"right-line\"><\/span> <\/div><\/div>[vc_column_text]<\/p>\n<ul>\n<li class=\"pdf\"><a href=\"https:\/\/www.prager-elektronik.at\/wp-content\/uploads\/OpenSPR-Brochure_digital.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">OpenSPR Datasheet<\/a><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<strong>What is Surface Plasmon Resonance (SPR)?<\/strong><\/p>\n<p>SPR is an optical effect that can be utilized to measure the binding kinetics and affinity of molecular interactions in real-time without the use of labels.<br \/>\nSPR is one of the few techniques that allows determination of binding kinetics k<sub>on<\/sub> and und k<sub>off<\/sub> as well as binding affinity K<sub>D<\/sub>.<\/p>\n<p><strong>How is LSPR different from SPR?<\/strong><\/p>\n<p>Localized surface plasmon resonance is generated by gold nanoparticles as compared to a continuous gold film as used in traditional SPR.<br \/>\nLSPR produces a strong resonance absorbance peak in the visible range of light, with it\u2019s position being highly<br \/>\nsensitive to the local refractive index surrounding the particle. Therefore, OpenSPR measures small changes in<br \/>\nthe wavelength of the absorbance position, rather than a reflected angle as in traditional SPR.<\/p>\n<p><strong>Advantages of using LSPR instead of SPR<\/strong><\/p>\n<ul>\n<li>The optical hardware for LSPR is less complex, so the instrument is smaller and more affordable<\/li>\n<li>Since the angle is not important, the instrument is more robust against vibration and mechanical noise<\/li>\n<li>LSPR is not as sensitive to bulk sample effects, which causes errors in experimental data, because it has a much shorter electromagnetic field decay length<\/li>\n<li>No strict temperature control is needed<\/li>\n<li>The sensor chips can be manufactured at a much more affordable price<\/li>\n<li>Instruments are easier to use and maintain<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column]<div class=\"title-wrapper  basel-title-color-default basel-title-style-default basel-title-size-small text-left \"><div class=\"liner-continer\"> <span class=\"left-line\"><\/span> <h4 class=\"title\" >Fields of Application<span class=\"title-separator\"><span><\/span><\/span><\/h4> <span class=\"right-line\"><\/span> <\/div><\/div>[vc_column_text]OpenSPR Systems are primarily used for affinity characterization respectively to determine Binding Kinetics of Proteins, Lipids, Carbohydrates, Antibodies, Nucleic Acids, Small Molecules, Cells, Viruses and Nanoparticles with the aim to use this data for Competition Assays, Target Identification, Epitope Mapping and for Screening in general.[\/vc_column_text]<div class=\"title-wrapper  basel-title-color-default basel-title-style-default basel-title-size-default text-center \"><div class=\"liner-continer\"> <span class=\"left-line\"><\/span> <h4 class=\"title\" >Accessories<span class=\"title-separator\"><span><\/span><\/span><\/h4> <span class=\"right-line\"><\/span> <\/div><\/div>[vc_tta_accordion style=&#8221;flat&#8221; c_icon=&#8221;triangle&#8221; active_section=&#8221;0&#8243; collapsible_all=&#8221;true&#8221;][vc_tta_section title=&#8221;OpenSPR Sensors&#8221; tab_id=&#8221;1587650354370-d3e29aa2-bad6&#8243;][vc_column_text]Our nanotechnology enabled sensors are manufactured to the highest quality to ensure consistent and repeatable measurements, at half of the price of traditional sensors.<br \/>\nWe achieve less than 2% CV on all critical optical properties.<br \/>\nOur sensors are stable in a variety of solvents, buffers and reagents.<br \/>\nWith high-quality and affordable OpenSPR Sensors, you have the freedom to run more experiments.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2571 alignright\" src=\"https:\/\/www.prager-elektronik.at\/wp-content\/uploads\/produkte_life-sciences_open-spr_chip-zugeschnitten2.jpg\" alt=\"\" width=\"300\" height=\"214\" \/><\/p>\n<p><strong>Available Materials:<\/strong><\/p>\n<p><strong>Carboxyl<\/strong> for any amine group using EDC\/NHS coupling<br \/>\n<strong>NTA<\/strong> for histidine tagged targets<br \/>\n<strong>Streptavidin<\/strong> for biotin tagged targets<br \/>\n<strong>Biotin<\/strong> for streptavidin coupled targets<br \/>\n<strong>Protein A<\/strong> for IgG based antibodies<br \/>\n<strong>Amine<\/strong> for any carboxyl group using EDC\/NHS coupling<br \/>\n<strong>Liposome Binding (LIP)<\/strong> for liposomes\/membrane proteins<br \/>\n<strong>Hydrophobic (MEM)<\/strong> for lipid monolayer<br \/>\n<strong>Thiol<\/strong> for thiol or maleimide tagged targets<br \/>\n<strong>Gold<\/strong> for non-functionalized and for custom surface chemistry development and targets with thiol groups[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Autosampler&#8221; tab_id=&#8221;1587650354392-d48620fa-1724&#8243;][vc_column_text]<strong>OpenSPR-XT &#8211; Accelerate your research with automated benchtop SPR.<\/strong><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2571 alignright\" src=\"https:\/\/www.prager-elektronik.at\/wp-content\/uploads\/2020\/04\/produkte_life-sciences_open-spr-xt.jpg\" alt=\"\" width=\"300\" height=\"214\" \/><br \/>\nOpenSPR-XT is built with our cutting-edge and affordable nanotechnology biosensor platform. It has been seamlessly integrated with our robust autosampler system to allow for fully automated, 24\/7 operation.<\/p>\n<p>Sample Capacity: 2x 96 well plates<br \/>\nTemperature Range: 4\u00b0C &#8211; 22\u00b0C[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][\/vc_column][\/vc_row]<\/p>\n<template id=\"brlbs-cmpnt-cb-template-google-maps-event-on\">\n <div class=\"brlbs-cmpnt-container brlbs-cmpnt-content-blocker\" data-borlabs-cookie-content-blocker-id=\"google-maps-event-on\" data-borlabs-cookie-content=\"\" ><div class=\"brlbs-cmpnt-cb-preset-b brlbs-cmpnt-cb-google-maps-event-on\"> <div class=\"brlbs-cmpnt-cb-thumbnail\" style=\"background-image: url('https:\/\/www.prager-elektronik.at\/wp-content\/uploads\/borlabs-cookie\/1\/brlbs-cb-google-maps-main.png')\"><\/div> <div class=\"brlbs-cmpnt-cb-main\"> <div class=\"brlbs-cmpnt-cb-content\"> <p class=\"brlbs-cmpnt-cb-description\">Sie sehen gerade einen Platzhalterinhalt von <strong>Google Maps<\/strong>. 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Bitte beachten Sie, dass dabei Daten an Drittanbieter weitergegeben werden.<\/p> <a class=\"brlbs-cmpnt-cb-provider-toggle\" href=\"#\" data-borlabs-cookie-show-provider-information role=\"button\">Mehr Informationen<\/a> <\/div> <div class=\"brlbs-cmpnt-cb-buttons\"> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-unblock role=\"button\">Inhalt entsperren<\/a> <a class=\"brlbs-cmpnt-cb-btn\" href=\"#\" data-borlabs-cookie-accept-service role=\"button\" style=\"display: inherit\">Erforderlichen Service akzeptieren und Inhalte entsperren<\/a> <\/div> <\/div> <\/div><\/div>\n<\/template>\n<script>\n(function() {\n        const template = document.querySelector(\"#brlbs-cmpnt-cb-template-facebook-content-blocker\");\n        const divsToInsertBlocker = document.querySelectorAll('div.fb-video[data-href*=\"\/\/www.facebook.com\/\"], div.fb-post[data-href*=\"\/\/www.facebook.com\/\"]');\n        for (const div of divsToInsertBlocker) {\n            const blocked = template.content.cloneNode(true).querySelector('.brlbs-cmpnt-container');\n            blocked.dataset.borlabsCookieContent = btoa(div.outerHTML);\n            div.replaceWith(blocked);\n        }\n})()\n<\/script><\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Publication-quality binding kinetics &amp; affinity data on your benchtop. 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