Zetasizer Nano ZS

The world’s most widely used system. It is used for the measurement of the size, electrophoretic mobility of proteins, zeta potential of colloids and nanoparticles, and optionally the measurement of protein mobility and microrheology of protein and polymer solutions. The high performance of the Zetasizer Nano ZS also enables the measurement of the molecular weight and second virial coefficient, A2, of macromolecules and kD, the DLS interaction parameter.

The system can also be used in a flow configuration to operate as a size detector for SEC or FFF.

The Zetasizer Nano ZS is a high performance two angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentration using dynamic light scattering with ‘NIBS’ optics. The ZSP also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer using static light scattering.

Using Non-Invasive Backscatter optics (NIBS) it has significantly better performance than systems using 90 degree scattering optics.

In addition, a microrheology option is available for measuring sample viscosity and viscoelastic properties, as well as a Protein Measurement option for protein mobility measurements.

The flow mode option enables the system to be connected to an SEC or an FFF system to use as a detector for the size of proteins or nanoparticles.

A choice of cuvettes are available, from disposable single-use to specific cells for viscous or high concentration samples or measuring the zeta potential of surfaces.

Parameters measured:
Particle and molecule size, translational diffusion, electrophoretic mobility, zeta potential of particles at high and low concentrations, viscosity and viscoelasticity of protein and polymer solutions, concentration, MW, A2, kD.

An optional accessory enables measurement of the zeta potential of solid surfaces.

  • Size measurement from 0.3nm (diameter) to 10 microns using patented NIBS (Non-Invasive Back Scatter) technology.
  • Zeta potential using patented M3-PALS technology.
  • Molecular weight measurement down to 980Da.
  • Microrheology option to measure viscosity and viscoelasticity.
  • Protein size measurement as dilute as 0.1mg/mL (Lysozyme).
  • Sample concentrations from 0.1ppm to 40%w/v.
  • Optional protein calculators, including protein charge, A2, kD, and molecular shape.
  • A ‘Quality Factor’ and ‘Expert Advice System’ gives the confidence of having an expert at your shoulder.
  • 21CFR part 11 software option to enable compliance with ER/ES.
  • Research software option to give access to further features and analysis algorithms for the light scattering specialist.
  • Automation of measurements using an autotitrator option.
  • Optional flow mode capability to enable use as a GPC / SEC size detector.
  • Alternative laser, 50mW at 532nm for samples incompatible with the standard 633nm laser.
  • Optical filter option to improve measurements with fluorescent sample.
  • Temperature range extension option to 120ºC.

Accessories

The MPT-2 is an accessory for the Zetasizer Nano that provides extensive automation for a number of measurements that are routinely required when characterizing the size and zeta potential of dispersed particles and molecules.

Both the size and zeta potential can be measured in sequence as part of the automated titration.

pH is one of the main influences of the zeta potential of materials, so measuring zeta potential as a function of pH, and the determination of the isoelectric point are often required. Automating this improves productivity by proving a complete measurement using a stored method.

Conductivity is also measured, and automation can speed up the determination of whether ions in a formulation are specifically or non-specifically bound.

The same automation can be extended to study the effect of the concentration of additives such as surfactants and polyelectrolytes, and there is a dilution function to study the effect of dilution on the size and zeta potential of the sample.

The Zetasizer NanoSampler can accommodate up to 96 sample vials, accurately delivering up to 3 sequential aliquots of each sample into the Zetasizer Nano for automated particle size or molecular size measurements. All operation remains within the easy-to-use Zetasizer software using the simple Standard Operating Procedure (SOP) interface. The NanoSampler is delivered complete with the software, analysis cell and fittings to attach to the Zetasizer Nano instrument.